人类小唾液腺间充质干细胞的自我更新和多能性特征  

Characterization of a Selfrenewing and Multipotent Cell Population Isolated from Human Minor Salivary Glands  

Authors: Lin Lu, Yan Li, Mingjuan Du, Chen Zhang, Xiangyu Zhang, Haizhou Tong, Lei Liu, TingJu Han, Wandi Li, Li Yan, Ningbei Yin, Haidong Li, Zhenmin Zhao  

摘要:  

成人干细胞在维持组织稳态中起重要作用。尽管这些细胞存在于许多组织中，但人类小唾液腺中干细胞的存在尚未得到充分探索。采用外植体培养方法，我们从小唾液腺中分离出一群具有自我更新和分化能力的细胞，称为人类小唾液腺间充质干细胞（hMSGMSCs）。这些细胞显示出胚胎干细胞和间充质干细胞的表型。我们的结果表明，hMSGMSCs在体外条件培养系统中具有分化为中胚层、外胚层和内胚层的潜力。此外，在部分肝切除术后将hMSGMSCs移植到SCID小鼠体内，结果显示hMSGMSCs能够存活并植入，表现为标记细胞的存活和肝细胞标志物AFP和KRT18的表达。这些数据证明了hMSGMSCs的存在，并表明其在细胞治疗和再生医学中的潜力。  

Abstract:

Adult stem cells play an important role in maintaining tissue homeostasis. Although these cells are found in many tissues, the presence of stem cells in the human minor salivary glands is not well explored. Using the explant culture method, we isolated a population of cells with selfrenewal and differentiation capacities harboring that reside in the human minor salivary glands, called human minor salivary gland mesenchymal stem cells (hMSGMSCs). These cells show embryonic stem cell and mesenchymal stem cell phenotypes. Our results demonstrate that hMSGMSCs have the potential to undergo mesodermal, ectodermal and endodermal differentiation in conditioned culture systems in vitro. Furthermore, in vivo transplantation of hMSGMSCs into SCID mice after partial hepatectomy shows that hMSGMSCs are able to survive and engraft, characterized by the survival of labeled cells and the expression of the hepatocyte markers AFP and KRT18. These data demonstrate the existence of hMSGMSCs and suggest their potential in cell therapy and regenerative medicine.